Effects of Keji Beling (Strobilanthes crispus) Crude Leaves Extract Against Aeromonas hydrophila Bacterial Infection in Vitro

Authors

Genesia Gerrine , Arief Prajitno , Mohamad Fadjar , Rahmalia Eka Kenitasari

DOI:

10.29303/jppipa.v9i5.3502

Published:

2023-05-31

Issue:

Vol. 9 No. 5 (2023): May

Keywords:

Antibacterial, Inhibition Test, Phytochemicals, SEM, Strobilanthes crispus

Research Articles

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How to Cite

Gerrine, G. ., Prajitno, A. ., Fadjar, M. ., & Kenitasari, R. E. . (2023). Effects of Keji Beling (Strobilanthes crispus) Crude Leaves Extract Against Aeromonas hydrophila Bacterial Infection in Vitro. Jurnal Penelitian Pendidikan IPA, 9(5), 3849–3855. https://doi.org/10.29303/jppipa.v9i5.3502

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Abstract

Keji beling leaves (Strobilanthes crispus) can be the solution to cure the fish that infected by Aeromonas hydrophila because it contained active compounds. The purpose of this study was to determine the active compounds contained in the crude extract of keji beling leaves (S. crispus) and their ability to inhibit the growth of A. hydrophila. The tests which are used in this study were phytochemical test, MIC test, disk diffusion test, and SEM test. Phytochemicals analysis showed the presence of active compounds are flavonoid, alkaloid, tanin, and triterpenoid. The result of MIC test showed that concentration of 100 mg/l with a value of 0.389 was the lowest absorbance of the keji beling (S. crispus) crude leaves extract. The inhibition test used disk diffusin test with five concentrations of keji beling (S. crispus) crude leaves extract with three replications: 50 mg/l, 100 mg/l, 150 mg/l, 200 mg/l, and 250 mg/l, with the observation time of 24 hours post-incubation and 48 hours post-incubation. The concentration of 250 mg/l had the highest inhibition zone. The result of SEM analysis showed that keji beling (S. crispus) crude leaves extract can damage the cell walls of A. hydrophila and lysis the bacterial cells.

References

Amanda, E. A., Oktiani, B. W., & Panjaitan, F. U. (2019). Efektivitas antibakteri ekstrak flavonoid propolis Trigona sp (Trigona thorasica) terhadap pertumbuhan bakteri Porphyromonas gingivalis. Dentin, 3(1), 23-28. https://doi.org/10.20527/dentin.v3i1.887

Amatulloh, Z., Mulyani, Y., Dewanti, L. P., & Haetami, K. (2021). Growth characteristics and tracing antagonistic properties of Bacillus flexus and Bacillus subtilis as antibacterials to overcome the attack of Aeromonas hydrophila bacteria on fish. World Scientific News, 158, 91-104. Retrieved from http://www.worldscientificnews.com/wp-content/uploads/2021/05/WSN-158-2021-91-104.pdf

Angelina, Maidiono, E., Putri, N. P., Arbianti, R., & Utami, T. S. (2019). Application of green extraction to increase the yield extract from Keji Beling (Strobilanthes crispus) leaves as hypercholesterolemia medicine. In AIP Conference Proceedings, 2092(1), 1-6. https://doi.org/10.1063/1.5096730

Behbahani, B. A., Noshad, M., & Falah, F. (2019). Cumin essential oil: Phytochemical analysis, antimicrobial activity and investigation of its mechanism of action through scanning electron microscopy. Microbial pathogenesis, 136, 1-6. https://doi.org/10.1016/j.micpath.2019.103716

Djamil, R., Pratami, D. K., & Riyantika, L. V. (2020). Pemeriksaan Parameter Mutu dan Uji Aktivitas Penghambatan Enzim α-Glukosidase dari Ekstrak Etanol 70% Daun Keji Beling (Sericocalyx Crispus (L.) Bremek). Jurnal Jamu Indonesia, 5(1), 1-8. https://doi.org/10.29244/jji.v5i1.181

Dwicahyani, T., Sumardianto, S., & Rianingsih, L. (2018). Uji Bioaktivitas Ekstrak Teripang Keling Holothuria atra Sebagai Antibakteri Staphylococcus aureus dan Escherichia coli. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 7(1), 15-24. Retrieved from https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/20384

Egra, S., Mardhiana, M., Rofin, M., Adiwena, M., Jannah, N., Kuspradini, H., & Mitsunaga, T. (2019). Aktivitas antimikroba ekstrak bakau (Rhizophora mucronata) dalam menghambat pertumbuhan Ralstonia solanacearum penyebab penyakit layu. Agrovigor: Jurnal Agroekoteknologi, 12(1), 26-31. https://doi.org/10.21107/agrovigor.v12i1.5143

Fardiyah, Q., Kurniawan, F., Ersam, T., & Slamet, A. (2020). Preliminary Phytochemical Screening and Fluorescence Characterization of Several Medicinal Plants Extract from East Java Indonesia. IOP Conference Series: Materials Science and Engineering, 833(1), 1-8. https://doi.org/10.1088/1757-899X/833/1/012008

Fera, O., Zaunit, M. M, & Mardatila, A. (2022). Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Markisa Konyal (Passiflora Ligularis Juss) Terhadap Staphylococcus Epidermidis Dan Escherichia Coli. Jurnal Katalisator, 7(2), 213-226. http://doi.org/10.22216/jk.v5i2.5717

Harlita, T. D., Oedjijono, & Asnani, A. (2018). The antibacterial activity of dayak onion (Eleutherine palmifolia (L.) merr) towards pathogenic bacteria. Tropical life sciences research, 29(2), 39-52. http://doi.org/10.21315/tlsr2018.29.2.4

Maftuch, M., Setyawan, F. H., & Suprastyani, H. (2018). Uji daya hambat ekstrak Chaetoceros calcitrans terhadap bakteri Aeromonas salmonicida. JFMR (Journal of Fisheries and Marine Research), 2(1), 39-46. https://doi.org/10.21776/ub.jfmr.2018.002.01.6

Maryani, R., Monalisa, S. S., & Rozik, M. (2018). In vitro test of natural antibacterial activity of yellow fruit moonseed Arcangelisia flava Merr. leaf on bacterium Pseudomonas fluorescens under different doses. AACL Bioflux, 11(1), 288-294. Retrieved from http://www.bioflux.com.ro/docs/2018.288-294

Najiya, U. L. (2022). Aktivitas Antibakteri Ekstrak Akar Jeruk Nipis (Citrus Aurantifolia) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli Dengan Metode Dilusi. Jurnal Kajian Ilmiah Kesehatan dan Teknologi, 4(2), 43-53. https://doi.org/10.52674/jkikt.v4i2.68

Ng, M. G., Ng, C. H., Ng, K. Y., Chye, S. M., Ling, A. P. K., & Koh, R. Y. (2021). Anticancer Properties of Strobilanthes crispus: A Review. Processes, 9(8), 1-18. https://doi.org/10.3390/pr9081370

Pambudi, A. R., Wasiaturrahmah, Y., & Aspriyanto, D. (2021). Antibacterial Effectiveness Of Kecapi Sentul Extract (Sandoricum Koetjape Merr.) Against Streptococcus Mutans. Odonto: Dental Journal, 8(2), 1-10. http://dx.doi.org/10.30659/odj.8.2.1-10

Prasetiowati, A. L., Prasetya, A. T., & Wardani, S. (2018). Sintesis Nanopartikel Perak dengan Bioreduktor Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi L.) Uji Aktivitasnya sebagai Antibakteri. Indonesian Journal of Chemical Science, 7(2), 160-166. https://doi.org/10.15294/ijcs.v7i2.20999

Putri, R., Supriyanta, J., & Adhil, D. A. (2021). Formulasi dan Uji Aktivitas Sediaan Masker Gel Peel Off Ekstrak Etanol 70% Daun Rambutan (Nephelium Lappaceum L.) Terhadap Propionibacterium Acnes. Journal of Pharmaceutical and Health Research, 2(1), 12-20. https://doi.org/10.47065/jharma.v2i1.836

Ruan, Z. H., Jiang, L. S., Li, Y. F., Lu, Z. Q., Zhang, X., & Liu, W. S. (2022). Transcriptomic analysis of the immune response against A. hydrophila infection in striped catfish (Pangasianodon hypophthalmus). Aquaculture, 547, 1-12. https://doi.org/10.1016/j.aquaculture.2021.737446

Saptiwi, B., Sunarjo, L., & Rahmawati, H. (2018). Perasan Jahe Merah (Zingiber officinale var. Rubrum) terhadap Daya Hambat Bakteri Aggregatibacter actinomycetemcomitans. Jurnal Riset Kesehatan, 7(2), 61-65. https://doi.org/10.31983/jrk.v7i2.3778

Sinurat, A. A. P., Renta, P. P., Herliany, N. E., Negara, B. F., & Purnama, D. (2019). Uji Aktivitas antibakteri ekstrak metanol rumput laut gracilaria edulis terhadap bakteri Aeromonas hydrophila. Jurnal Enggano, 4(1), 105-114. https://doi.org/10.31186/jenggano.4.1.105-114

Sofidiana, L. L., Sulistyani, E., & Lestari, P. E. (2022). Daya Hambat Kombinasi Ekstrak Pegagan (Centella asiatica, L.) dan Peppermint terhadap Pertumbuhan Streptococcus mutans. Pustaka Kesehatan, 10(3), 195-201. https://doi.org/10.19184/pk.v10i3.25232

Stratev, D., Zhelyazkov, G., Noundou, X. S., & Krause, R. W. (2018). Beneficial effects of medicinal plants in fish diseases. Aquaculture International, 26, 289-308. https://doi.org/10.1007/s10499-017-0219-x

Sulistiyono, F. D., Sofihidayati, T., & Lohitasari, B. (2018). Uji aktivitas antibakteri dan fitokimia kulit bawang merah (Allium cepa L.) hasil ekstraksi metode microwave assisted extraction (MAE). Mandala Of Health, 11(2), 71-79. https://doi.org/10.20884/1.mandala.2018.11.2.1316

Wantenia, F., Susanto, C., & Suksestio, M. (2020). Pengaruh Strobilanthes crispus BI terhadap KHM dan KBM pada bakteri Aggregatibacter actinomyecetemcomitans dan Fusobacterium nucleatum secara in vitro. Jurnal Ilmiah dan Teknologi Kedokteran Gigi, 16(1), 36-44. https://doi.org/10.52674/jkikt.v4i2.68

Yustiati, A., Kundari, D. F., Suryana, A. H., & Suryadi, I. B. B. (2019). Effectiveness of potassium diformate addition to feed to improve immune system of Pangasius (Pangasianodon hypophthalmus) that challenged by Aeromonas hydrophila. World Scientific News, 134(2), 86-100. Retrieved from http://www.worldscientificnews.com/wp-content/uploads/2019/06/WSN-1342-2019-86-100.pdf

Author Biographies

Genesia Gerrine, University of Brawijaya

Arief Prajitno, University of Brawijaya

Mohamad Fadjar, University of Brawijaya

Rahmalia Eka Kenitasari, University of Brawijaya

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Copyright (c) 2023 Genesia Gerrine Lucia Tampubolon

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